Advanced Fluorescence Reporters in Chemistry and Biology II: - download pdf or read online

By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)

ISBN-10: 3642046991

ISBN-13: 9783642046995

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“This e-book is a accomplished evaluate of the different sorts of nanoscale probes which have been and are at the moment being built for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for growth of the cloth coated. … a invaluable source that covers the factitious, photo-physical, and theoretical points of this diversified box of research.” (Jeffrey T. Petty, magazine of the yankee Chemical Society, Vol. 133, 2011)

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Extra resources for Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles

Example text

The report of several established and recent methods for extracellular and intracellular labeling of biomolecules, in conjunction with some commercial tools for these applications [99] is mainly advantageous for organic fluorophores. This includes several strategies for site-specific covalent and noncovalent labeling of biomolecules, typically proteins, in living cells. Examples are enzyme-catalyzed labeling by posttranslational modification, as in biotin ligase-catalyzed introduction of biotin into biotin acceptor peptides, which may be used to label proteins at the cell surface.

5), to measure the distance between donor and acceptor chromophores (spectroscopic ruler, monitoring of conformational changes), for the design of ratiometric probes and sensors as well as signal amplification strategy [117, 120]. Typically, donor and acceptor chromophores are chemically different (hetero-FRET or donor–acceptor energy transfer (DAET). , to sense the protein aggregation state based on steady state and time-resolved measurements of the fluorescence anisotropy [117]. FRET applications thus require labeling of biomolecules or other targets with one donor and one acceptor group (heteroFRET) or with a single class of chromophores (homo-FRET).

The cytotoxicity of heavy metals or rare-earth elements, which are present in many nanocrystals as core and shell materials, is well known. Thus, it is critical to know whether these cytotoxic substances can leak out of the nanocrystals over time. This may happen upon illumination or oxidation [89]. Furthermore, toxic ligands or coatings might be released into solution [85]. Some groups found that CdSe-based QDs were cytotoxic to cells [90], other did not detect cytotoxic reactions [91]. In cases where cytotoxicity was observed, it was attributed to leaking of cytotoxic elements, cytotoxic surface ligands, and/or nanoparticle aggregation.

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Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles by Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)


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