A Guidebook to Lipoprotein Technique - download pdf or read online

By R.H. BURDON and P.H. van KNIPPENBERG (Eds.)

ISBN-10: 0444805745

ISBN-13: 9780444805744

ISBN-10: 0444805915

ISBN-13: 9780444805911

ISBN-10: 0720442001

ISBN-13: 9780720442007

This quantity is a accomplished choice of equipment for the isolation, characterization, research and estimation of soluble lipoproteins. It surveys each one point of lipoprotein know-how in a serious demeanour with a view to let the investigator to pick the tools so much proper to his standards. Having made his selection, he'll then discover a specific description of the way to accomplish the method.

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Sample text

0257 g/ml at 26 "C. 03 g/ml. 0364 g/ml. Unfortunately, we do not at present have the information with which to compensate accurately for this differential expansivity in all centrifugations. In the first place, the LDL are the only lipoproteins - Ch. 2 ISOLATION AND PURIFICATION OF PLASMA LIPOPROTEINS 23 for which any attempt to determine the thermal expansivity has been made. At present therefore, a correction can only be applied to VLDL and HDL if it is assumed that their coefficients of expansivity are the same as that for LDL.

I . Isolation by ultracentrifugation This technique separates the lipoproteins according to differences in their hydrated density. The most widely used procedure is the serial centrifugation at different solvent densities that originated in the Donner laboratory and has been described in exquisite detail by Lindgren (1975), although it is to Have1 et al. (1955) that the popularity of the method is probably due. Although the technique is not well suited to the fractionation of very large quantities of plasma, it offers the best combination of capacity and resolution that is available.

This time can be considerably reduced by running at 50000 rev/min, when the field at the top of the tube amounts to 149 000 g. 2Ti or 60Ti can only be used in centrifuges of the appropriate rating. If the machines available are not rated to handle these high energy rotors, it may be necessary to use others such as the Beckman 30 or 40 series. The Beckman 35 rotor (total volume 564 ml) can be used to prepare VLDL or LDL, but is inadequate for the isolation of HDL. For this purpose, the 40 rotor (total volume 162 46 A GUIDEBOOK TO LIPOPROTEIN TECHNIQUE ml) can be used.

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A Guidebook to Lipoprotein Technique by R.H. BURDON and P.H. van KNIPPENBERG (Eds.)


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